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mitotracker red cmxros staining solution  (Beyotime)


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    Structured Review

    Beyotime mitotracker red cmxros staining solution
    Mitotracker Red Cmxros Staining Solution, supplied by Beyotime, used in various techniques. Bioz Stars score: 99/100, based on 458 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mitotracker+red+cmxros+staining+solution/Hoechst+33342+Staining+Solution/pm42015210-123-34-50
    Average 99 stars, based on 458 article reviews
    mitotracker red cmxros staining solution - by Bioz Stars, 2026-10
    99/100 stars

    Images

    Related Articles

    Membrane:

    Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline
    Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Hoechst 33342 staining solution were sequentially added (Beyotime, Shanghai, China). ..

    Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline.
    Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Hoechst 33342 staining solution were sequentially added (Beyotime, Shanghai, China). ..

    Binding Assay:

    Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline
    Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Hoechst 33342 staining solution were sequentially added (Beyotime, Shanghai, China). ..

    Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline.
    Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Hoechst 33342 staining solution were sequentially added (Beyotime, Shanghai, China). ..

    Staining:

    Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline
    Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Hoechst 33342 staining solution were sequentially added (Beyotime, Shanghai, China). ..

    Article Title: Multi-tissue analysis identifies mitochondrial genes in chicken aging-induced productivity decline.
    Article Snippet: .. Following the instructions of the mitochondrial membrane potential and apoptosis detection kit with MitoTracker Red CMXRos and Annexin V-FITC, 188 μL of Annexin V-FITC binding solution, 5 μL of Annexin V-FITC, 2 μL of MitoTracker Red CMXRos staining solution, and 5 μL of Hoechst 33342 staining solution were sequentially added (Beyotime, Shanghai, China). ..

    Article Title: Ndufa8 promotes white fat Browning by improving mitochondrial respiratory chain complex I function to ameliorate obesity by in vitro and in vivo.
    Article Snippet: Obesity and its complications have become a global health problem that needs to be addressed urgently.. White adipose tissue (WAT) browning contributes to consuming excess energy in WAT, which is important for improving obesity and maintaining a healthy energy homeostasis.. Mitochondria, as the energy metabolism center of cells, are extensively involved in many metabolic processes, including the browning of WAT.

    Article Title: Establishment and identification of the gill cell line from the blunt snout bream (Megalobrama amblycephala) and its application in studying gill remodeling under hypoxia.
    Article Snippet: To probe the mechanisms of gill remodeling in blunt snout bream under hypoxic conditions, we selected gill tissue for primary cell culture to establish and characterize the first blunt snout bream gill cell line, named MAG.. The gill cells were efficiently passaged in M199 medium supplemented with 8% antibiotics and 15% fetal bovine serum at 28 °C, exhibiting primarily an epithelial-fibroblast mixed type.. Additionally, the MAG cells (17th generation) were subjected to four experimental conditions—normoxia, hypoxia 12 h, hypoxia 24 h, and reoxygenation 24 h (R24h)—to evaluate the effects of hypoxia and reoxygenation on MAG cells during gill remodeling.

    Incubation:

    Article Title: Ndufa8 promotes white fat Browning by improving mitochondrial respiratory chain complex I function to ameliorate obesity by in vitro and in vivo.
    Article Snippet: Obesity and its complications have become a global health problem that needs to be addressed urgently.. White adipose tissue (WAT) browning contributes to consuming excess energy in WAT, which is important for improving obesity and maintaining a healthy energy homeostasis.. Mitochondria, as the energy metabolism center of cells, are extensively involved in many metabolic processes, including the browning of WAT.

    other:

    Article Title: MicroRNA-224-5p nanoparticles balance homeostasis via inhibiting cartilage degeneration and synovial inflammation for synergistic alleviation of osteoarthritis.
    Article Snippet: MitoTracker Red CMXRos apoptosis assay Chondrocytes were co-cultured with 193 μL Annexin V-FITC ixture (green), 2 μl Mito-Tracker Red CMXRos staining solution red) and 20 μL 4, 6-diamidino-2-phenyindole dilactate (DAPI, Beytime Biotechnology, China) staining solution (blue) at 20-25 ◦C for 0 min in the dark.



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    Image Search Results


    L-carnitine (Lca) maintained fatty acid oxidation (FAO) and mitochondrial homeostasis under lipopolysaccharide (LPS) stimulation in THP-1-derived macrophages. A , Effects of Lca on PPAR, LXR, and ABCA1 mRNA expression in macrophages upon LPS stimulation. B , Fluorescence images showing the impact of Lca on mitochondrial integrity (stained with MitoTracker Red probe, shown in red) in THP-1 derived macrophages. Scale bar: 50 μm. C and D , Impact of Lca on LPS-induced intracellular and mitochondrial (mt) reactive oxygen species (ROS) production in macrophages using DCFH-DA ( C ) and MitoSOX ( D ) fluorescence assay, respectively. E , Immunoblotting and related quantitative analysis of OPA1 and MFN2 for mitochondrial fusion, as well as DRP1 and FIS1 for mitochondrial fission in THP-1-derived macrophages. Data are reported as means±SE (n=3-4). *P<0.05, **P<0.01, ***P<0.001, ns: not significant ( A - D ). # P<0.05, ## P<0.01 vs Control group; *P<0.05, **P<0.01 vs LPS group ( E ). Statistical analysis was performed using one-way ANOVA followed by Bonferroni's post hoc test.

    Journal: Brazilian Journal of Medical and Biological Research

    Article Title: L-carnitine reduces acute lung injury via mitochondria modulation and inflammation control in pulmonary macrophages

    doi: 10.1590/1414-431X2023e12830

    Figure Lengend Snippet: L-carnitine (Lca) maintained fatty acid oxidation (FAO) and mitochondrial homeostasis under lipopolysaccharide (LPS) stimulation in THP-1-derived macrophages. A , Effects of Lca on PPAR, LXR, and ABCA1 mRNA expression in macrophages upon LPS stimulation. B , Fluorescence images showing the impact of Lca on mitochondrial integrity (stained with MitoTracker Red probe, shown in red) in THP-1 derived macrophages. Scale bar: 50 μm. C and D , Impact of Lca on LPS-induced intracellular and mitochondrial (mt) reactive oxygen species (ROS) production in macrophages using DCFH-DA ( C ) and MitoSOX ( D ) fluorescence assay, respectively. E , Immunoblotting and related quantitative analysis of OPA1 and MFN2 for mitochondrial fusion, as well as DRP1 and FIS1 for mitochondrial fission in THP-1-derived macrophages. Data are reported as means±SE (n=3-4). *P<0.05, **P<0.01, ***P<0.001, ns: not significant ( A - D ). # P<0.05, ## P<0.01 vs Control group; *P<0.05, **P<0.01 vs LPS group ( E ). Statistical analysis was performed using one-way ANOVA followed by Bonferroni's post hoc test.

    Article Snippet: After 24 h, cells were incubated with MitoTracker Red CMXRos (MTR) staining solution (Invitrogen, M7512).

    Techniques: Derivative Assay, Expressing, Fluorescence, Staining, Western Blot

    Journal: Cell Reports Medicine

    Article Title: PARP-1 improves leukemia outcomes by inducing parthanatos during chemotherapy

    doi: 10.1016/j.xcrm.2023.101191

    Figure Lengend Snippet:

    Article Snippet: Cells were seeded in 6-well plates at a density of 5 x 10 5 /2 mL media per well and incubated with 1 μM (OCI-AML2) or 10 μM (OCI-AML3) of ara-C for 24 h. Cells were transferred to centrifuge tubes and stained with 500 μL Mitotracker Red CMXRos staining solution (200 nM in PBS, Thermo Fisher Scientific) for 30 min at 37°C.

    Techniques: Recombinant, Derivative Assay, Staining, Negative Control, Plasmid Preparation, Caspase-3 Assay, TUNEL Assay, Reverse Transcription, Expressing, Bicinchoninic Acid Protein Assay, Western Blot, Microarray, Software, Fluorescence